Comments; The use of 18 GHz EMF at a specific absorption rate (SAR) of 3 kW/kg & 5 kW/kg is really a high power output; for somebody who weights 100 kg (a large man) that would require 300,000 to 500,000 Watts of power—like standing right in front of a commercial large radio transmitter.  The fact that this much power causes changes in the cell membrane is not surprising.


The mechanisms by which various biological effects are triggered by exposure to an electro-

magnetic field are not fully understood and have been the subject of debate. Here, the effects

of exposing typical representatives of the major microbial taxa to an 18 GHz microwave electro-

magnetic field (EMF)were studied. It appeared that the EMF exposure induced cell permeabili-

sation in all of the bacteria and yeast studied, while the cells remained viable (94% throughout

the exposure), independent of the differences in cell membrane fatty acid and phospholipid

composition. The resulting cell permeabilisation was confirmed by detection of the uptake of

propidium iodine and 23 nm fluorescent silica nanospheres using transmission electron

microscopy (TEM) and confocal laser scanning microscopy (CLSM). Upon EMF exposure, the

bacterial cell membranes are believed to become permeable through quasi-endocytosis pro-

cesses. The dosimetry analysis revealed that the EMF threshold level required to induce the

uptake of the large (46 nm) nanopsheres was between three and six EMF doses, with a spe-

cific absorption rate (SAR) of 3 kW/kg and 5 kW/kg per exposure, respectively, depending on

the bacterial taxa being studied. It is suggested that the taxonomic affiliation and lipid composi-

tion (e.g. the presence of phosphatidyl-glycerol and/or pentadecanoic fatty acid) may affect the

extent of uptake of the large nanospheres (46 nm). Multiple 18 GHz EMF exposures over a

one-hour period induced periodic anomalous increases in the cell growth behavior of two

Staphylococcus aureus strains, namely ATCC 25923 and CIP 65.8T.


Dr. Raymond Oenbrink
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