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Rosetta Stone for HLA Haplotyping |
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Labcorp: ACC 20001604256 HLA DRB, DQ Typing Labcorp Test #; 167120 |
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DRB1 |
DQ |
DRB3 |
DRB4 |
DRB5 |
Clinical |
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Multisusceptible |
4 |
3 |
53 |
Severe RA, Malaria, Autoimmune hepatitis 3% |
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11/12 |
3 |
52B |
Vaccine, athletes tall/hypermobile 1% |
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14 |
5 |
52B |
0.1% incidence |
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Mold |
7 |
2/3 |
53 |
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13 |
6 |
52A,B,C |
0.05% incidence |
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17 |
2 |
52A |
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18* |
4 |
52A |
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Borrelia, post Lyme Syndrome |
15 |
6 |
51 |
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16 |
5 |
51 |
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Dinoflagellates |
4 |
7/8 |
53 |
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Ciguatera |
4 |
3 |
53 |
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11 |
3 |
52B |
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Multiple Antibiotic Resistant Staph epidermidis (MARCoNS) |
11 |
7 |
52B |
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Low MSH |
1 |
5 |
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No recognized significance |
8 |
3,4,6 |
53 |
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Low-risk Mold |
7 |
9 |
53 |
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12 |
7 |
52B |
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9 |
9 |
53 |
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Rosetta Stone Interpretation: |
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1 |
Look at the LabCorp report. There are five categories of line entries: |
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DRB1 |
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DQ |
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DRB3 |
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DRB4 |
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DRB5 |
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2 |
Each individual will have two sets of three alleles; |
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Unless the DRB1 is 1, 8 or 10. |
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i. Those patients will only have a DQ. |
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ii. These won't have DRB 3, 4, 5. |
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3 |
Each individual with a DRB1 other than 1, 8 or 10 will have a DQ and one other allele from DRB 3, 4, 5 |
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4 |
1. If you are expecting to find two entries in, say DR 3, 4, 5 but only find one, the patient is homozygous for that allele and only one allele will appear on the PCR. Don't be worried that the numbers and letters look impossible to understand. They just have to be translated. |
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5 |
You will translate these categories into; |
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B1 |
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DQ |
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B3 |
52 (ABC) |
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B4 |
53 &... |
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B5 |
51 respectively |
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If the translation were easy and made sense, you wouldn't need this |
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6 |
The numbers and letters in each of the 5 categories are given to an excessive amount of detail. Write down only the first two numbers in each line. |
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7 |
DRB1; When you see 03 as one of the two genes, an allele, for DRB1, rewrite it as 17. |
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8 |
Record the entries in B3 by converting the 01 to A, the 02 to B and the 03 to C; this will give you 52A, 52B and 52C, respectively: |
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B4 is 53 |
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B5 is 51 |
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9 |
Record the genotypes in two columns, one each representing each parent, using the templates in the appendix. |
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1st Parent |
DRB1-DQ-DR(3,4,5) |
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2nd Parent |
DRB1-DQ-DR(3,4,5) |
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